Prom1^{tm1(cre/ERT2)Gilb}
Targeted Allele Detail

Summary

• Symbol: Prom1^{tm1(cre/ERT2)Gilb}
• Name: prominin 1; targeted mutation 1, Richard J Gilbertson
• MGI ID: MGI:3830624
• Synonyms: CD133-CreER^T2, Prom1^C-L
• Gene: Prom1 Location: Chr5:44150962-44259374 bp, - strand Genetic Position: Chr5, 23.97 cM
• Alliance: Prom1^{tm1(cre/ERT2)Gilb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144215
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Intragenic deletion
• Prom1^{tm1(cre/ERT2)Gilb} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Prom1 (MGI:1100886)

• Mutation details: The ATG start site of the gene locus was replaced by a cassette containing a creERT2 fusion gene, an IRES-LacZ gene, and a FRT-flanked neomycin selection cassette. Correct targeting was confirmed by PCR. The neomycin selection cassette was later removed by crossing mice with Gt(ROSA)26Sor^tm1(FLP1)Dym mice. Beta-gal expression was detected in the neural tube, rib, kidney, and gut of E10.5-E16.5 heterozygote embryos. Beta-gal expression was also detected in numerous adult tissues including brain, kidney, retina, pancreas, and intestine. Cre expression was also detected in brain, kidney, lung, pancreas, and intestinal epithelium of heterozygote adult mice after tamoxifen administration. Inactivation of the endogenous gene locus was confirmed by RT-PCR of adult homozygous kidney. (J:144215)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Prom1 (mouse) Summary of all recombinase alleles driven by Prom1. Alliance mouse Prom1 gene page

Tumor Data

List all tumor models in MMHCdb carrying Prom1^{tm1(cre/ERT2)Gilb}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prom1 Mutation: 76 strains or lines available

References

• Original: J:144215 Zhu L, et al., Prominin 1 marks intestinal stem cells that are susceptible to neoplastic transformation. Nature. 2009 Jan 29;457(7229):603-7
• All: 33 reference(s)