Hprt1^{tm4(Ple88-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm4(Ple88-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 4, Elizabeth M Simpson
• MGI ID: MGI:3830628
• Synonyms: Hprt1^{tm4(GFAP-EGFP)Ems}, Hprt1^{tm4(mEMS1671)Ems}, Hprt1^{tm4(Ple88-EGFP;mEMS1671)Ems}, Ple88;GFAP-A;mEMS1671
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm4(Ple88-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS1661, mEMS1667, mEMS1671, mEMS1672
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple88-EGFP transgene (pEMS1375) was designed with a Ple88 minipromoter (GFAP-A; a 2210 bp promoter region of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  In most cells, EGFP immunoreactivity fills the cell bodies and processes; revealing their full morphology and indicating the glial nature of the cell types. There is EGFP expression in A): astroglial-like cells in the forebrain and cortex, hippocampus and alvius, the cerebellar white matter, as well as the midbrain, brainstem, and eye, B): nascent neuronal/glial progenitor cells in the developing forebrain (including rostral cortical subventricular zone and the rostral migratory stream, and C): numerous cells in the optic nerve and the proximal segment as the nerve enters the retina (much like typical astrocytic staining). While EGFP and GFAP co-expression is observed, not all EGFP+ cells are GFAP+; reflecting the possibility that the Ple88 minipromoter may express in non-glial cell types. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1671 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)