Hprt1^{tm7(Ple185-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm7(Ple185-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 7, Elizabeth M Simpson
• MGI ID: MGI:3830629
• Synonyms: Hprt1^{tm7(mEMS1627)Ems}, Hprt1^{tm7(Ple185-EGFP;mEMS1627)Ems}, Ple185;S100B-C;mEMS1627, S100B-EGFP
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm7(Ple185-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS1603, mEMS1604, mEMS1610, mEMS1627
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple185-EGFP transgene (pEMS1384) was designed with a Ple185 minipromoter (S100beta-C; a 2440 bp promoter segment and 1340 bp basal promoter element, both from the human S100 calcium binding protein B (S100B) promoter region) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  The promoter/regulatory regions of the human S100 calcium binding protein B (S100B) gene directs expression of enhanced green fluorescent protein (EGFP) to neural white matter glia and astrocytes. There is broad EGFP expression throughout the brain in some glial populations, most notably in cerebellar Bergmann glia, as well as fiber tracts throughout the brain. Descending white matter fiber tracts coursing through the corpus callosum, striatum, and into the brainstem are also prominently labeled. The EGFP expression pattern in the brain co-labels with myelinated axons in the cortex, striatum, and brainstem. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1627 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)