Siae^tm1.1Avrk
Targeted Allele Detail

Summary

• Symbol: Siae^tm1.1Avrk
• Name: sialic acid acetylesterase; targeted mutation 1.1, Ajit Varki
• MGI ID: MGI:3830709
• Synonyms: Siae^flox
• Gene: Siae Location: Chr9:37525117-37559554 bp, + strand Genetic Position: Chr9, 20.79 cM
• Alliance: Siae^tm1.1Avrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144035
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutations: Insertion, Intragenic deletion Mutation details: This locus can generate two alternatively spliced variants that encode either a lysosomal sialic acid acetylesterase (Lse) or a cytosolic esterase (Cse). Exon 2 is unique to the Lse transcript, encoding the N-terminus of the protein product. A floxed neomycin resistance cassette was placed upstream of exon 2 and an additional loxP site was placed downstream of exon 2. Transient cre expression in correctly targeted ES cells removed the neomycin selection cassette leaving exon 2 flanked by loxP sites. (J:144035)

Generation of the Siae^tm1.1Avrk and Siae^tm1.2Avrk alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Siae Mutation: 33 strains or lines available

References

• Original: J:144035 Cariappa A, et al., B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase. J Exp Med. 2009 Jan 16;206(1):125-38
• All: 1 reference(s)