Etv2^{Gt(141.1H7)Cmhd}
Gene trapped Allele Detail

Summary

• Symbol: Etv2^{Gt(141.1H7)Cmhd}
• Name: ets variant 2; gene trap 141.1H7, Centre for Modeling Human Disease
• MGI ID: MGI:3830713
• Synonyms: ER71/Etv2 knockout, Etsrp71^-, Etv2^{Gt(CMHD-GT_141.1H7-3)Cmhd}
• Gene: Etv2 Location: Chr7:30333041-30335277 bp, - strand Genetic Position: Chr7, 18.92 cM, cytoband A3
• Alliance: Etv2^{Gt(141.1H7)Cmhd} page

Absence of an endocardial/endothelial lineage in Etv2^{Gt(141.1H7)Cmhd}/Etv2^{Gt(141.1H7)Cmhd} heart and embryo

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: 141.1H7
• Germline Transmission: Earliest citation of germline transmission: J:143866
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: Gep-SD5
• Mutation details: The gene trap vector pGep-SD5 was inserted at the exon intron boundary of exon 5. The absence of transcript expression was confirmed by RT-PCR on heart extracts. (J:143866)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CMHD-GT_141.1H7-3	CL706334 ; (MGI Seq Detail)	3' RACE	unknown
  * GRCm39; (strand of gene trap mutagenesis)

Schematic of the construct used to generate the Etv2^{Gt(141.1H7)Cmhd} allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Etv2 Mutation: 14 strains or lines available

References

• Original: J:143866 Ferdous A, et al., Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo. Proc Natl Acad Sci U S A. 2009 Jan 20;106(3):814-9
• All: 8 reference(s)