Ptcd2^{Gt(RRF537)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Ptcd2^{Gt(RRF537)Byg}
• Name: pentatricopeptide repeat domain 2; gene trap RRF537, BayGenomics
• MGI ID: MGI:3830811
• Gene: Ptcd2 Location: Chr13:99456157-99481215 bp, - strand Genetic Position: Chr13, 52.76 cM, cytoband D1
• Alliance: Ptcd2^{Gt(RRF537)Byg} page

Mutation origin

• Mutant Cell Line: RRF537
• Germline Transmission: Earliest citation of germline transmission: J:143946
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector containing a beta-geo cassette was inserted downstream of exon 8. The absence of full-length transcript was confirmed by northern blot analysis. (J:143946)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRF537	CC325981 ; (MGI Seq Detail)	5' RACE	Chr13:99463145-99479861 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptcd2 Mutation: 25 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:143946 Xu F, et al., Disruption of a mitochondrial RNA-binding protein gene results in decreased cytochrome b expression and a marked reduction in ubiquinol-cytochrome c reductase activity in mouse heart mitochondria. Biochem J. 2008 Nov 15;416(1):15-26
• All: 2 reference(s)