Igh^tm1Leck
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Leck
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Laurel A Eckhardt
• MGI ID: MGI:3830835
• Synonyms: VHdelta, VHdelta^a
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144033
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Inserted expressed sequence, Modified regulatory region, Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A 3.7-kb region (including the most proximal D-heavy gene segment, DQ52, all the J-heavy gene segments, and the Emu enhancer element (Rr150)) was replaced with a loxP-flanked neomycin resistance gene cassette and a fully assembled V-heavy gene segment. The V-heavy gene segment and about 2 kb of upstream sequence were isolated from the B1-8 hybridoma, which produces a 4-hydroxy-3-nitrophenyl acetyl-binding antibody. Founder mice were crossed with EIIA-cre transgenic mice to remove the neomycin cassette from the germline. Correct targeting was confirmed by Southern blot and genomic PCR. Homozygous mice had B cells expressing the knocked in immunoglobulin heavy chain. (J:144033)

Generation of the Igh^tm1Leck allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:144033 Li F, et al., A role for the IgH intronic enhancer E mu in enforcing allelic exclusion. J Exp Med. 2009 Jan 16;206(1):153-67
• All: 3 reference(s)