Fgf2^tm3Doe
Targeted Allele Detail

Summary

• Symbol: Fgf2^tm3Doe
• Name: fibroblast growth factor 2; targeted mutation 3, Thomas Doetschman
• MGI ID: MGI:3831082
• Synonyms: Fgf2^hmw-ko, FGF2(Lo)
• Gene: Fgf2 Location: Chr3:37402616-37464255 bp, + strand Genetic Position: Chr3, 18.41 cM, cytoband A2-B
• Alliance: Fgf2^tm3Doe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144353
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using a modified Tag & Exchange targeting scheme, the Hprt minigene in the tagged Fgf2 knock-out allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a 14 bp oligonucleotide designed to produce stop codons in all three reading frames and to cause a frameshift in translation products originating from the noncanonical CTG initiation codon. Cells with the exchanged allele were selected for the loss of HPRT function using 6-thioguanine. The loss of high molecular weight protein product expression was confirmed by western blot analysis on heart, liver and brain extracts. The low molecular weight protein product is still expressed. (J:144353)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgf2 Mutation: 19 strains or lines available

References

• Original: J:144353 Azhar M, et al., Gene targeted ablation of high molecular weight fibroblast growth factor-2. Dev Dyn. 2009 Feb;238(2):351-7
• All: 9 reference(s)