En2^tm7.1Alj
Targeted Allele Detail

Summary

• Symbol: En2^tm7.1Alj
• Name: engrailed 2; targeted mutation 7.1, Alexandra L Joyner
• MGI ID: MGI:3831095
• Synonyms: En2^GFPloxIRES
• Gene: En2 Location: Chr5:28370692-28377164 bp, + strand Genetic Position: Chr5, 13.94 cM
• Alliance: En2^tm7.1Alj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:156169
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was generated by inserting an EGFP-IRES-LoxP-Frt sequence into the 5' UTR of the En2 locus immediately upstream of the ATG and a LoxP site into the intron. An Frt-flanked neo cassette was removed by Flp mediated recombination.

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	En1^tm1Alj/En1^tm8.1Alj En2^tm1Alj/En2^tm7.1Alj Gt(ROSA)26Sor^tm1(cre/ERT2)Alj/Gt(ROSA)26Sor^+	involves: 129S1/Sv * 129S2/SvPas * 129S6/SvEvTac * 129X1/SvJ * Swiss Webster
cx2	En1^tm1Alj/En1^tm8.1Alj En2^tm1Alj/En2^tm7.1Alj	involves: 129S1/Sv * 129S2/SvPas * 129S6/SvEvTac * 129X1/SvJ * Swiss Webster

Phenotypes:

Affected Systems	cn1	cx2
show or hide all annotated terms
nervous system	√	√
abnormal cerebellar foliation	√	√
abnormal cerebellum hemisphere lobule morphology	√	√
abnormal cerebellum vermis lobule morphology	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any En2 Mutation: 105 strains or lines available

References

• Original: J:156169 Cheng Y, et al., The Engrailed homeobox genes determine the different foliation patterns in the vermis and hemispheres of the mammalian cerebellum. Development. 2010 Feb;137(3):519-29
• All: 3 reference(s)