Ncor1^tm1Laz
Targeted Allele Detail

Summary

• Symbol: Ncor1^tm1Laz
• Name: nuclear receptor co-repressor 1; targeted mutation 1, Mitchell A Lazar
• MGI ID: MGI:3831119
• Synonyms: DADm, NcoR1^Y478A, N-DADm
• Gene: Ncor1 Location: Chr11:62207132-62348200 bp, - strand Genetic Position: Chr11, 38.08 cM, cytoband B2
• Alliance: Ncor1^tm1Laz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142342
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A Y478A mutation was introduced into exon 13 of the locus, which is within the deacetylase activation domain of the protein product. A floxed neomycin selection cassette was also placed about 500 bp downstream from the targeted exon. Founder mice derived from correctly targeted ES cells were crossed with EIIa-cre transgenic mice to removed the neomycin selection cassette. The introduced mutation was predicted to disrupt interactions between the Ncor1 protein product and the histone deacetylase Hdac3. Expression of the mutant allele was similar to endogenous expression as determined by immunoblot analysis of homozygous liver extracts. The mutant Ncor1 protein failed to interact with Hdac3 protein as determined by immunoprecipitation assays. (J:142342)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncor1 Mutation: 396 strains or lines available

Notes

ES cells from Chemicon

References

• Original: J:142342 Alenghat T, et al., Nuclear receptor corepressor and histone deacetylase 3 govern circadian metabolic physiology. Nature. 2008 Dec 18;456(7224):997-1000
• All: 11 reference(s)