Gt(ROSA)26Sor^{tm4(Wnt7a)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(Wnt7a)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Andrew P McMahon
• MGI ID: MGI:3831175
• Synonyms: R26-C-Wnt7aIL, R26-Wnt7aIRESLacZ, ROSA26-CAGGS-Wnt7a
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(Wnt7a)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142352
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(Wnt7a)Amc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Wnt7a (MGI:98961)

• Mutation details: A cassette containing a splice acceptor, the puromycin resistance gene, and a poly A sequence was placed in front of a CAGGS promoter. Downstream of the promoter was placed a floxed terminator element containing three SV40 poly A sequences (tpA) followed by a bicistronic cassette encoding for Wnt7a and IRES-nlacZ. In the absence of Cre recombinase, transcription from the CAGGS enhancer/promoter is terminated by the tpA element. In the presence of cre recombinase, the tpA is recombined out and the CAGGS enhancer/promoter drives expression of both Wnt7a and betagalactosidase. Ectopic Wnt7a and lacZ expression were detected in the neuroepithelium of developing embryos when this allele was on a Tg(Nes-cre)1Kln transgenic background. (J:142352)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:142352 Stenman JM, et al., Canonical Wnt signaling regulates organ-specific assembly and differentiation of CNS vasculature. Science. 2008 Nov 21;322(5905):1247-50
• All: 1 reference(s)