F7^{tm1(tTA,tetO-F7)Edr}
Targeted Allele Detail

Summary

• Symbol: F7^{tm1(tTA,tetO-F7)Edr}
• Name: coagulation factor VII; targeted mutation 1, Elliot D Rosen
• MGI ID: MGI:3831192
• Synonyms: F7^tm1Edr, FVII^tTA
• Gene: F7 Location: Chr8:13076034-13085809 bp, + strand Genetic Position: Chr8, 5.73 cM
• Alliance: F7^{tm1(tTA,tetO-F7)Edr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144540
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence, Reporter, Transactivator)
• Mutations: Insertion, Intragenic deletion
• F7^{tm1(tTA,tetO-F7)Edr} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	F7 (MGI:109325)

• Mutation details: The tetracycline transactivator (tTA) coding sequence followed by an IRES and lacZ coding sequence such were inserted downstream of the promoter region such that the ATG codon of tTA was inserted at the initiation site. In addition a construct with the tTA responsive promoter (phcmv-1) driving expression of an F7 cDNA was inserted adjacent to the lacZ containing cassette and this was followed by a floxed neo cassette. This total construct replaced the entire coding sequence of the gene. Analysis indicates that protein activity in plasma from homozygotes is about 0.7% that of wild-type controls. Results indicate that tetracycline treatment does not significantly change expression. (J:144540)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F7 Mutation: 28 strains or lines available

References

• Original: J:144540 Rosen ED, et al., Generation of genetically-altered mice producing very low levels of coagulation factorVII. Thromb Haemost. 2005 Sep;94(3):493-7
• All: 4 reference(s)