Nfatc1^tm3Glm
Targeted Allele Detail

Summary

• Symbol: Nfatc1^tm3Glm
• Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1; targeted mutation 3, Laurie H Glimcher
• MGI ID: MGI:3831720
• Synonyms: Nfatc1^fl
• Gene: Nfatc1 Location: Chr18:80649420-80756286 bp, - strand Genetic Position: Chr18, 53.66 cM, cytoband E4
• Alliance: Nfatc1^tm3Glm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144598
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site replaced 3 kb of intronic sequence upstream of exon 3 and a floxed neomycin cassette replaced was placed downstream of the exon into a XbaI site. Mice derived from correctly targeted ES cells were crossed with EIIa-cre transgenic mice and progeny were selected by Southern blotting for selective loss of the neomycin resistance cassette. Protein expression was unaffected as determined by immunoblotting of splenocyte extracts. Cre-mediated excision of exon 3 will remove a regulatory domain necessary for proper protein function. (J:144598)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfatc1 Mutation: 49 strains or lines available

References

• Original: J:144598 Aliprantis AO, et al., NFATc1 in mice represses osteoprotegerin during osteoclastogenesis and dissociates systemic osteopenia from inflammation in cherubism. J Clin Invest. 2008 Nov;118(11):3775-89
• All: 28 reference(s)