Gt(ROSA)26Sortm3(HIF1A*)Kael
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm3(HIF1A*)Kael |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 3, William G Kaelin |
MGI ID: |
MGI:3832385 |
Synonyms: |
Hif-1alphaLSL, ROSA26-HA-HIF1dPA |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm3(HIF1A*)Kael page
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Germline Transmission: |
Earliest citation of germline transmission:
J:144666
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Parent Cell Line: |
TC1/TC-1 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence) |
Mutations: |
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Insertion, Nucleotide substitutions
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Gt(ROSA)26Sortm3(HIF1A*)Kael expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
HIF1A (3091) |
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Mutation details: DNA encoding influenza virus hemagglutinin A- (HA-) tagged human hypoxia-inducible factor 1 alpha (HIF1alpha) with nucleotide substitutions leading to replacement of proline by alanine at amino acid positions 402 and 564 (P402A;P564A), preceded by a floxed neo-STOP cassette, was inserted into the targeted locus. The STOP sequence must be removed by Cre-mediated recombination to allow for expression of the mutated coding sequence. The amino acid substitutions prevent oxygen-dependent prolyl hydroxylation at a.a. position 402 or 564, which is required for binding by von Hippel-Lindau tumor suppressor (VHL).
(J:144666)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:144666 Kim WY, et al., Failure to prolyl hydroxylate hypoxia-inducible factor alpha phenocopies VHL inactivation in vivo. EMBO J. 2006 Oct 4;25(19):4650-62 |
All: |
20 reference(s) |
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