Lyn^mld4
Chemically induced Allele Detail

Summary

• Symbol: Lyn^mld4
• Name: LYN proto-oncogene, Src family tyrosine kinase; multilineage defect 4
• MGI ID: MGI:3832561
• Synonyms: Lyn^kd
• Gene: Lyn Location: Chr4:3676865-3791613 bp, + strand Genetic Position: Chr4, 2.05 cM
• Alliance: Lyn^mld4 page

Mutation origin

• Strain of Origin: B6.129S1-Mpl^tm1Wsa

Mutation description

• Allele Type: Chemically induced (ENU)
• Mutation: Single point mutation
• Mutation details: ENU mutagenesis of mice carrying the Mpl^tm1Wsa allele caused a C-to-A mutation in the 25th nucleotide pair of exon 12. This mutation is predicted to cause the substitution of a lysine for a threonine at residue 429 in the highly conserved Src activation loop of the protein product. Founder mice were bred with wild-type mice to breed away the Mpl^tm1Wsa allele. Mutant protein isolated from the B cells of homozygous mice was found to lack kinase activity in an in vitro transkinase assay. (J:144799)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lyn Mutation: 65 strains or lines available

References

• Original: J:144799 Verhagen AM, et al., A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiency. J Immunol. 2009 Feb 15;182(4):2020-9
• All: 2 reference(s)