Chrna9^tm1Elgo
Targeted Allele Detail

Summary

• Symbol: Chrna9^tm1Elgo
• Name: cholinergic receptor, nicotinic, alpha polypeptide 9; targeted mutation 1, A Belen Elgoyhen
• MGI ID: MGI:3832679
• Synonyms: Chrna9^L9'T
• Gene: Chrna9 Location: Chr5:66092264-66134669 bp, + strand Genetic Position: Chr5, 33.84 cM
• Alliance: Chrna9^tm1Elgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144698
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: The leucine at position 210 was mutated to a theonine (L210T) by changing the corresponding codon in exon 4 from TTG toACG. A floxed neomycin cassette was inserted into the NcoI restriction site within intron 3 for targeting purposes and was subsequently removed by crossing founder mice with cre transgenic mice. Quantitative PCR demonstrated expression levels of the mutant allele were similar to the wild-type allele. (J:144698)

Generation of the Chrna9^tm1Elgo allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrna9 Mutation: 40 strains or lines available

References

• Original: J:144698 Taranda J, et al., A point mutation in the hair cell nicotinic cholinergic receptor prolongs cochlear inhibition and enhances noise protection. PLoS Biol. 2009 Jan 20;7(1):e18
• All: 6 reference(s)