Lipe^tm2Rze
Targeted Allele Detail

Summary

• Symbol: Lipe^tm2Rze
• Name: lipase, hormone sensitive; targeted mutation 2, Rudolf Zechner
• MGI ID: MGI:3832721
• Gene: Lipe Location: Chr7:25078952-25097911 bp, - strand Genetic Position: Chr7, 13.78 cM, cytoband A3
• Alliance: Lipe^tm2Rze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74644
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin selection cassette was inserted in intron 1 and an additional loxP site was inserted 3' to exon 7. The resulting targeted ES cells were subjected to transient cre expression. The neomycin selection cassette was deleted leaving exons 2 through 7 flanked by loxP sites. In addition to this allele, transient expression provided recombinant ES cells in which exons 2-7 were also deleted. These cells were used to produce Lipe^tm1Rze. (J:74644)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lipe Mutation: 37 strains or lines available

References

• Original: J:74644 Haemmerle G, et al., Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis. J Biol Chem. 2002 Feb 15;277(7):4806-15
• All: 5 reference(s)