Plcg2^tm1Ichi
Targeted Allele Detail

Summary

• Symbol: Plcg2^tm1Ichi
• Name: phospholipase C, gamma 2; targeted mutation 1, Hirotake Ichise
• MGI ID: MGI:3833397
• Synonyms: Plcg2^EGFP, PLCgamma2-null
• Gene: Plcg2 Location: Chr8:118225030-118361881 bp, + strand Genetic Position: Chr8, 64.26 cM
• Alliance: Plcg2^tm1Ichi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:143529
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/SvEv and C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of the coding region of exon 2 was replaced with EGFP cDNA followed by a murine PGK polyadenylation signal sequence and an FRT-flanked PGK-gb2-neo cassette. Newborn heart tissue lysates from homozygous mutant animals did not contain protein product as determined by Western blot. (J:143529)

Expression

In Mice Carrying this Mutation:	39 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plcg2 Mutation: 74 strains or lines available

Notes

The ES cell line used to generate this allele was established from Plcg2⁺/Plcg2^al blastocysts. All correctly targeted clones possessed the Plcg2⁺/Plcg2^tm1Ichi genotype.

References

• Original: J:143529 Ichise H, et al., Phospholipase C{gamma}2 is necessary for separation of blood and lymphatic vasculature in mice. Development. 2009 Jan;136(2):191-5
• All: 1 reference(s)