Gt(ROSA)26Sor^{tm1(Tcfe2a/Tal1)Xhsu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Tcfe2a/Tal1)Xhsu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Xiao-Hong Sun
• MGI ID: MGI:3833549
• Synonyms: ET2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Tcfe2a/Tal1)Xhsu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144569
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A selection marker and a transcriptional stop cassette were flanked by loxP sites and placed upstream of a chimeric gene. This chimeric gene encodes the transactivation domains of Tcfe2a (E47) and the bHLH domain of Tal1 (SCL). An IRES-GFP cassette was placed downstream of the chimeric gene and the whole construct (loxP-neo-STOP-loxp- Tcfe2a/Tal1-IRES-GFP) was knocked into the locus. Upon expression of cre recombinase, the chimeric protein and GFP will be expressed, with the chimeric protein predicted to have a dominant negative effect on members of the Id and Tal protein family due to its strong affinity for E transcription factors. (J:144569)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:144569 Cochrane SW, et al., Balance between Id and E proteins regulates myeloid-versus-lymphoid lineage decisions. Blood. 2009 Jan 29;113(5):1016-26
• All: 3 reference(s)