Pold1^tm1Uchi
Targeted Allele Detail

Summary

• Symbol: Pold1^tm1Uchi
• Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 1, Arikuni Uchimura
• MGI ID: MGI:3833573
• Synonyms: Pold1^-
• Gene: Pold1 Location: Chr7:44182168-44198239 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Pold1^tm1Uchi page

Impaired proliferation of Pold1^tm1Uchi/Pold1^tm1Uchi blastocysts

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144992
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: An A to C transversion in exon 10 causes an aspartic acid to alanine amino acid residue change at codon 400. A floxed fragment containing the SV40 splice/polyadenylation signal as well as a neomycin resistance cassette was inserted in intron 10. Western blot analysis of extracts prepared from E12.5 heterozygous whole embryos confirmed that protein product was reduced by about half. (J:144992)

Generation of Pold1^tm1Uchi and Pold1^tm1.1Uchi alleles

Expression

In Mice Carrying this Mutation:	5 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pold1 Mutation: 63 strains or lines available

References

• Original: J:144992 Uchimura A, et al., DNA polymerase delta is required for early mammalian embryogenesis. PLoS One. 2009;4(1):e4184
• All: 1 reference(s)