Mep1a^tm1Bond
Targeted Allele Detail

Summary

• Symbol: Mep1a^tm1Bond
• Name: meprin 1 alpha; targeted mutation 1, Judith S Bond
• MGI ID: MGI:3833886
• Gene: Mep1a Location: Chr17:43785215-43813703 bp, - strand Genetic Position: Chr17, 19.73 cM, cytoband C1-D1
• Alliance: Mep1a^tm1Bond page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145147
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette with an in-frame stop codon was inserted into the zinc-catalytic center encoded by exon 7. In the process, 140 bp were deleted at the targeting site. End-point PCR of kidney mRNA demonstrated the absence of transcription. Absence of protein product was indicated by immunoblot analysis of urine. (J:145147)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mep1a Mutation: 47 strains or lines available

References

• Original: J:145147 Banerjee S, Meprin Metalloproteases Modulate Intestinal Host Response. PhD Thesis - Pennsylvania State University. 2008
• All: 15 reference(s)