Ppp1r15b^tm1Dron
Targeted Allele Detail

Summary

• Symbol: Ppp1r15b^tm1Dron
• Name: protein phosphatase 1, regulatory subunit 15B; targeted mutation 1, David Ron
• MGI ID: MGI:3833920
• Gene: Ppp1r15b Location: Chr1:133058904-133067538 bp, + strand Genetic Position: Chr1, 57.8 cM
• Alliance: Ppp1r15b^tm1Dron page

Stunted growth of Ppp1r15b^tm1Dron/Ppp1r15b^tm1Dron mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144954
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A portion of exon 1, which contains the translation initiation methionine and codes for the first 416 amino acids, was replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on extracts of untreated mouse embryonic fibroblasts (MEFs) or MEFs treated with tunicamycin. (J:144954)

Generation of the Ppp1r15b^tm1Dron allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppp1r15b Mutation: 14 strains or lines available

References

• Original: J:144954 Harding HP, et al., Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2alpha) dephosphorylation in mammalian development. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1832-7
• All: 3 reference(s)