Tg(Myh6-Pln)416Egk
Transgene Detail

Summary

• Symbol: Tg(Myh6-Pln)416Egk
• Name: transgene insertion 416, Evangelia G Kranias
• MGI ID: MGI:3834110
• Synonyms: Tg(Pln)416Egk, TgPLN^wt
• Transgene: Tg(Myh6-Pln)416Egk Location: unknown
• Alliance: Tg(Myh6-Pln)416Egk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-Pln)416Egk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Pln (MGI:97622)

• Mutation details: The transgene comprises a wild-type mouse phospholamban cDNA that contains 13 bp of 5' untranslated sequence, the entire coding region and 434 bp of 3' untranslated sequence, including the first endogenous polyadenylation site, followed by an SV40 polyadenylation signal and driven by the 5.5 kb cardiac-specific alpha myosin heavy chain (myosin, heavy polypeptide 6, cardiac muscle, alpha) promoter. Northern blot analysis revealed cardiac expression of the 1.0 kb transcript from the transgene, and quantitative immunoblot analysis demonstrated overexpression of phospholamban protein in the hearts of transgenic mice. (J:135899)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:135899 Kadambi VJ, et al., Cardiac-specific overexpression of phospholamban alters calcium kinetics and resultant cardiomyocyte mechanics in transgenic mice. J Clin Invest. 1996 Jan 15;97(2):533-9
• All: 3 reference(s)