Hbb-y^tm2.2Fier
Targeted Allele Detail

Summary

• Symbol: Hbb-y^tm2.2Fier
• Name: hemoglobin Y, beta-like embryonic chain; targeted mutation 2.2, Steven Fiering
• MGI ID: MGI:3834620
• Synonyms: deltaEy(inv)
• Gene: Hbb-y Location: Chr7:103500961-103502414 bp, - strand Genetic Position: Chr7, 54.85 cM
• Alliance: Hbb-y^tm2.2Fier page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145362
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion, Inversion
• Hbb-y^tm2.2Fier involves 3 genes/genome features (Hbb-bh1, Hbb-bh0, Hbb-y) View all
• Mutation details: 1.1 kb of DNA containing the promoter region, exon1, and exon2 was replaced with a floxed PGK-hygro selection cassette in ES cells already containing the targeted allele Hbb-bh1 allele. The selection cassettes in the two genes were subsequently excised by breeding mice to CMV-cre transgenic mice. Because the two loxP sites left behind are in reverse orientation to one another, cre also induced an inversion of the DNA segment between the two sites. This allele is only found in conjunction with Hbb-bh1. (J:145362)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbb-y Mutation: 4 strains or lines available

References

• Original: J:145362 Hu X, et al., Transcriptional interference among the murine beta-like globin genes. Blood. 2007 Mar 1;109(5):2210-6
• All: 1 reference(s)