Tcra^Ln3Jae
Allele Detail

Summary

• Symbol: Tcra^Ln3Jae
• Name: T cell receptor alpha chain; recombination line 3, Rudolf Jaenisch
• MGI ID: MGI:3834626
• Synonyms: LN3alpha
• Gene: Tcra Location: Chr14:52665424-54461655 bp, + strand Genetic Position: Chr14, cytoband D1-D2

Mutation origin

• Strain of Origin: (129S4/SvJae x C57BL/6)F1

Mutation description

• Allele Type: Not Applicable
• Mutation: Other
• Mutation details: This allele was created by transfer of a male alpha-beta T cell nucleus into an enucleated oocyte. A blastocyte was grown and from this an ES cell clone was derived. The two Tcra alleles in this ES cell were recombined with one allele having a productive, in frame recombination and the other a non-productive, out of frame recombination. Only the productive allele containing a Valpha3.1-J30 recombination was propagated and is the one described here. Recombination was determined by Southern blotting and inverted PCR. (J:143006)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcra Mutation: 101 strains or lines available

References

• Original: J:143006 Serwold T, et al., Early TCR expression and aberrant T cell development in mice with endogenous prerearranged T cell receptor genes. J Immunol. 2007 Jul 15;179(2):928-38
• All: 3 reference(s)