Tcrb^Ln3Jae
Allele Detail

Summary

• Symbol: Tcrb^Ln3Jae
• Name: T cell receptor beta chain; recombination line 3, Rudolf Jaenisch
• MGI ID: MGI:3834628
• Synonyms: LN3beta, Vbeta14^NT
• Gene: Tcrb Location: Chr6:40868230-41535305 bp, + strand Genetic Position: Chr6, cytoband A-C

Mutation origin

• Strain of Origin: (129S4/SvJae x C57BL/6)F1

Mutation description

• Allele Type: Not Applicable
• Mutation: Other
• Mutation details: This allele was created by transfer of a male alpha-beta T cell nucleus into an enucleated oocyte. A blastocyte was grown and from this an ES cell clone was derived. The two Tcra alleles in this ES cell were recombined with one a productive, in frame recombination and the other a non-productive, out of frame recombination. Only the productive allele containing a Vbeta1-D1-J1.4 recombination was propagated and is the one described here. Recombination was determined by Southern blotting and inverted PCR. (J:143006)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tcrb Mutation: 97 strains or lines available

References

• Original: J:143006 Serwold T, et al., Early TCR expression and aberrant T cell development in mice with endogenous prerearranged T cell receptor genes. J Immunol. 2007 Jul 15;179(2):928-38
• All: 7 reference(s)