Kdm1a^tm1.1Tche
Targeted Allele Detail

Summary

• Symbol: Kdm1a^tm1.1Tche
• Name: lysine (K)-specific demethylase 1A; targeted mutation 1.1, Taiping Chen
• MGI ID: MGI:3834629
• Synonyms: Aof2^2lox
• Gene: Kdm1a Location: Chr4:136277851-136330034 bp, - strand Genetic Position: Chr4, 68.8 cM
• Alliance: Kdm1a^tm1.1Tche page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144527
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Germline, cre mediated recombination was used to remove the beta-geo cassette leaving exons 10 through 13 floxed. Subsequent molecular analysis revealed that two adenine-to-guanine point mutations were inadvertently introduced into the tower domain during the gene targeting process. These resulted in the predicted amino acid changes E413G and M448V in the encoded protein. These residues have been implicated in protein-protein interactions with other regulatory proteins. Microarray and qRT-PCR analysis demonstrated that mRNA levels were decreased by ~50% in E18.5 homozygous hearts, indicating a hypomorphic allele. (J:144527, J:270116)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm1a Mutation: 39 strains or lines available

References

• Original: J:144527 Wang J, et al., The lysine demethylase LSD1 (KDM1) is required for maintenance of global DNA methylation. Nat Genet. 2009 Jan;41(1):125-9
• All: 2 reference(s)