Pak1^tm1Cher
Targeted Allele Detail

Summary

• Symbol: Pak1^tm1Cher
• Name: p21 (RAC1) activated kinase 1; targeted mutation 1, Jonathan Chernoff
• MGI ID: MGI:3834675
• Gene: Pak1 Location: Chr7:97437748-97561588 bp, + strand Genetic Position: Chr7, 53.57 cM
• Alliance: Pak1^tm1Cher page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:142439
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector replaced the coding sequence of the p21-binding domain and the inhibitory domain with a neomycin resistance gene in the anti-sense orientation. A lack of protein was confirmed in homozygote bone marrow-derived mast cells by immunoblot assays. (J:142439, J:145444)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pak1 Mutation: 37 strains or lines available

References

• Original: J:142439 McDaniel AS, et al., Pak1 regulates multiple c-Kit mediated Ras-MAPK gain-in-function phenotypes in Nf1+/- mast cells. Blood. 2008 Dec 1;112(12):4646-54
• All: 31 reference(s)