Ext1^tm1Vcs
Targeted Allele Detail

Summary

• Symbol: Ext1^tm1Vcs
• Name: exostosin glycosyltransferase 1; targeted mutation 1, Val C Sheffield
• MGI ID: MGI:3834913
• Synonyms: EXT1, Ext1^e2neofl, Ext1^tm1Kjns
• Gene: Ext1 Location: Chr15:52931657-53209579 bp, - strand Genetic Position: Chr15, 20.0 cM
• Alliance: Ext1^tm1Vcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145524
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An inverted loxP site was introduced upstream to exon 2 in a genomic framgment containing the Ext1 gene. The fragment was then subcloned into a vector upstream of the forward-facing loxP-flanked neomycin resistance (Neo) cassette. The linearized targeti vector was electroporated into R1 ES cells. Cre-mediated recombination of this allele will result in inversion of exon2 of Ext1 and distruption of Ext1 function. (J:145524, J:157599)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ext1 Mutation: 65 strains or lines available

References

• Original: J:145524 Sheffield VC, Generation of a floxed allele of Ext1. MGI Direct Data Submission. 2009
• All: 7 reference(s)