Gt(ROSA)26Sor^{tm1(RNAi:Bmpr2)Dliu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(RNAi:Bmpr2)Dliu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Dong Liu
• MGI ID: MGI:3835030
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(RNAi:Bmpr2)Dliu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145514
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Knockdown, RMCE-ready)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(RNAi:Bmpr2)Dliu} involves 1 genes/genome features (Bmpr2) View all
• Mutation details: A transcriptional stop signal was inserted upstream of a hygromycin cassette which flanked upstream by lox511 and downstream by a loxP site in reverse orientation. The resulting properly targeted ES cells were then used for RCME (recombinase mediated cassette exchange). The exchange cassette contained a lox522 site followed in order by a U6 PolIII promoter, and shRNA duplex corresponding to a 19-mer in exon 10 of Bmpr2, a neomycin selection cassette, and finally a reverse orientation loxP site. Cre mediated cassette exchange was then performed in ES cells. (J:145514)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:145514 Liu D, et al., Dosage-dependent requirement of BMP type II receptor for maintenance of vascular integrity. Blood. 2007 Sep 1;110(5):1502-10
• All: 1 reference(s)