Tg(DBH-PTPN11)33Bmas
Transgene Detail

Summary

• Symbol: Tg(DBH-PTPN11)33Bmas
• Name: transgene insertion 33, Brian A Masters
• MGI ID: MGI:3835396
• Synonyms: DN33, TgN(DBH-SHP2^C459S)Bmas33
• Transgene: Tg(DBH-PTPN11)33Bmas Location: unknown
• Alliance: Tg(DBH-PTPN11)33Bmas page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(DBH-PTPN11)33Bmas expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  chicken	PTPN11 (395815)

• Mutation details: This transgene contains a 1.9 kb cDNA encoding a catalytically inactive mutant chicken protein tyrosine phosphatase, non-receptor type 11 (also known as SHP-2) in which the cysteine at amino acid position 459 is replaced by serine. The cDNA resides downstream of a 5.8 kb human dopamine beta-hydroxylase (dopamine beta-monooxygenase) promoter followed by the first intron of the rat insulin II gene and is joined at its 3' end to a 799 bp fragment of the human growth hormone gene that contains 66 bp of coding sequence and the 633 bp 3' untranslated region (UTR), including the polyadenylation signal. Immunoblot analysis reveals that superior cervical ganglia of transgenic mice contain 2-2.5 fold the level of DBH as do those of non-transgenic animals. (J:80747)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This is one of three lines that expressed the catalytically inactive C459S mutant PTNP11: lines 21, 31 and 33. The phenotype is reported to be consistent among them. (J:80747)

References

• Original: J:80747 Chen B, et al., SHP-2 Mediates Target-Regulated Axonal Termination and NGF-Dependent Neurite Growth in Sympathetic Neurons. Dev Biol. 2002 Dec 15;252(2):170-87
• All: 1 reference(s)