Dock8^pri
Chemically induced Allele Detail

Summary

• Symbol: Dock8^pri
• Name: dedicator of cytokinesis 8; primurus
• MGI ID: MGI:3835421
• Synonyms: Dock8^S1827P
• Gene: Dock8 Location: Chr19:24976898-25179796 bp, + strand Genetic Position: Chr19, 19.91 cM
• Alliance: Dock8^pri page

Mutation origin

• Strain of Origin: C57BL/6JAnu
• Project Collection: APF ENU Mutagenesis

Mutation description

• Allele Type: Chemically induced (ENU)
• Mutation: Single point mutation
• Mutation details: This mutation was discovered in an ENU mutagenesis screen for mutations that do not interfere with the initial antibody response to an immunogen, but prevent its maturation and persistence. The mutation comprises a T to C transition that replaces an absolutely conserved serine at amino acid position 1827, in the DOCK-homology region 2 (DHR2) domain of the protein, with proline (S1827P). DHR2 is known to bind to G proteins of the Rho family, promoting GTP --> GDP exchange and initiating a variety of cellular responses. The S1827P substitution is predicted to disrupt the structure of DHR2 lobe B alpha-helix 6 and thus to interfere with its GTP --> GDP exchange function. (J:155516)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Dock8 Mutation: 103 strains or lines available

References

• Original: J:155516 Randall KL, et al., Dock8 mutations cripple B cell immunological synapses, germinal centers and long-lived antibody production. Nat Immunol. 2009 Dec;10(12):1283-91
• All: 8 reference(s)