Hprt1^{tm10(Ple162-EGFP/cre)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm10(Ple162-EGFP/cre)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 10, Elizabeth M Simpson
• MGI ID: MGI:3835521
• Synonyms: Hprt1^{tm10(mEMS954)Ems}, Hprt1^{tm10(Ple162-EGFPcre;mEMS954)Ems}, PITX3-EGFP/cre
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm10(Ple162-EGFP/cre)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS951, mEMS953, mEMS954
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Hprt1^{tm10(Ple162-EGFP/cre)Ems} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  PITX3 (5309)

  Pitx3

  (MGI:1100498)

• Mutation details: The Ple162-EGFPCre transgene (pEMS1148) was designed with the 3636 bp Ple162 minipromoter (PITX3-D; derived from an intronic candidate regulatory region and a segment of the human paired-like homeodomain 3 (PITX3) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). (J:164356)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	PITX3 (human) Summary of all recombinase alleles driven by PITX3. Comparative matrix view of recombinase activities Alliance human PITX3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS954 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 2 reference(s)