Hprt1^{tm15(Ple111-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm15(Ple111-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 15, Elizabeth M Simpson
• MGI ID: MGI:3835527
• Synonyms: HCRT-EGFP, Hprt1^{tm15(mEMS1774)Ems}, Hprt1^{tm15(Ple111-EGFP;mEMS1774)Ems}, Ple111
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm15(Ple111-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS1767, mEMS1768, mEMS1774, mEMS1775
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple111-EGFP transgene (pEMS1417) was designed with the 544 bp Ple111 minipromoter (HCRT-A; derived from a subsection of the promoter from the human hypocretin (orexin) neuropeptide precursor (HCRT)) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  The promoter/regulatory regions of the human hypocretin (orexin) neuropeptide precursor (HCRT) directs expression of an enhanced green fluorescent protein (EGFP) to a small cluster of EGFP-positive neurons is found in the hypothalamus. EGFP-immunoreactivity fills the cytoplasm of these cells, highlighting the cell body as well as extensive dendritic processes. This label is very specific to the hypothalamus and not seen in any other cell populations in the brain. Double label immunocytochemistry shows that a majority of these EGFP-positive hypothalamic cells co-express with the HCRT (orexin) neuropeptide (the gene from which this MiniPromoter was designed). (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1774 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)