Hprt1^{tm18(Ple90-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm18(Ple90-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 18, Elizabeth M Simpson
• MGI ID: MGI:3835531
• Synonyms: GFAP-EGFP, Hprt1^{tm18(mEMS1899)Ems}, Hprt1^{tm18(Ple90-EGFP;mEMS1899)Ems}, Ple90
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm18(Ple90-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS1889, mEMS1899, mEMS1900, mEMS1903
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The Ple90-EGFP transgene (pEMS1377) was designed with the 1433 bp Ple90 minipromoter (GFAP-C; derived from a putative upstream regulatory region and a portion of the promoter of the human glial fibrillary acidic protein (GFAP) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  The promoter/regulatory regions of the human glial fibrillary acidic protein (GFAP) gene directs broad expression of enhanced green fluorescent protein (EGFP) in glial cellular profiles throughout the brain and spinal cord. Numerous astroglial profiles are seen in the cortical grey and white matter and in the cerebellum the full extent of Bergmann glial processes are highlighted with anti-EGFP immunocytochemistry. EGFP-immunoreactivity is most prominent in the cell bodies across all regions, with a subpopulation of cells showing a halo of process staining. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS1899 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)