Tg(Scgb1a1-rtTA,tetO-Il13)1Eli
Transgene Detail

Summary

• Symbol: Tg(Scgb1a1-rtTA,tetO-Il13)1Eli
• Name: transgene insertion 1, Jack A Elias
• MGI ID: MGI:3835944
• Synonyms: CC10-rtTA-IL-13, iIl-13
• Transgene: Tg(Scgb1a1-rtTA,tetO-Il13)1Eli Location: unknown
• Alliance: Tg(Scgb1a1-rtTA,tetO-Il13)1Eli page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F2

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Scgb1a1-rtTA,tetO-Il13)1Eli expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Il13 (MGI:96541)

• Mutation details: The CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivation (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-IL-13-hGH construct contains a polymeric tetracycline operator (tet-O), minimal CMV promoter, murine IL-13 cDNA, and hGH intronic and polyadenylation signals. Both constructs were coinjected into fertilized oocytes, and dual positive founder animals were obtained. In the presence of doxycycline (dox), rtTA is able to bind in trans to the tet-O, and the VP-16 transactivator activates IL-13 gene transcription. In the absence of dox, rtTA binding occurs at very low levels, and only low level gene transcription is noted. (J:131678)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:131678 Zheng T, et al., Inducible targeting of IL-13 to the adult lung causes matrix metalloproteinase- and cathepsin-dependent emphysema. J Clin Invest. 2000 Nov;106(9):1081-93
• All: 19 reference(s)