Tmpo^tm1.1Foi
Targeted Allele Detail

Summary

• Symbol: Tmpo^tm1.1Foi
• Name: thymopoietin; targeted mutation 1.1, Roland Foisner
• MGI ID: MGI:3835992
• Synonyms: Lap2alpha^-
• Gene: Tmpo Location: Chr10:90983433-91017177 bp, - strand Genetic Position: Chr10, 45.66 cM
• Alliance: Tmpo^tm1.1Foi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145628
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: At least six alternative transcripts can be derived from this gene locus. This mutation targeted exon 4, which is necessary for generation of a protein product that localizes to the nucleoplasm. A loxP site and a FRT-flanked neomycin cassette was placed upstream of exon 4 and an additional loxP site was placed downstream of the exon. Founder mice were crossed with Cre-deleter mice to remove exon 4 and the neomycin cassette. Homozygote mice lacked the nucleoplasm specific protein isoform but expressed the other protein isoforms as determined by immunoblot analysis of heart and intestinal extracts. (J:145628)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmpo Mutation: 39 strains or lines available

References

• Original: J:145628 Naetar N, et al., Loss of nucleoplasmic LAP2alpha-lamin A complexes causes erythroid and epidermal progenitor hyperproliferation. Nat Cell Biol. 2008 Nov;10(11):1341-8
• All: 5 reference(s)