Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli
Transgene Detail

Summary

• Symbol: Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli
• Name: transgene insertion 1, Jack A Elias
• MGI ID: MGI:3836057
• Synonyms: CC10-rtTA-tTS-TGF-beta1 Tg, CC10-tTS-rtTA-TGF-beta1 Tg, TgN(CC10-rtTA)-TgN(tTS)-TgN(TGF-b1)
• Transgene: Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli Location: unknown
• Alliance: Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Scgb1a1-rtTA,-tTS,tetO-TGFB1*)1Eli expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TGFB1 (7040)

  Tgfb1

  (MGI:98725)

• Mutation details: The CC10-rtTA-hGH construct contains the CC10 promoter, the reverse tetracycline transactivation (rtTA), and human growth hormone (hGH) intronic and polyadenylation sequences. The tet-O-CMV-Tgfb1-SV40, contains a polymeric tetracycline operator (tet-O), minimal cytomegalovirus (CMV) promoter, human Tgfb1 cDNA in which the cysteine codons at positions 223 and 225 were replaced with serine codons, and SV40 polyadenylation signals. The third construct, CC10-tTS-hGH, has the CC10 promoter driving the expression of the tetracycline-controlled transcriptional silencer (tTS). All three constructs were coinjected into fertilized oocytes, and triple positive founder animals were obtained. In this system, the CC10 promoter constitutively drives the expression of rtTA and tTS in a lung-specific fashion. In the absence of dox, tTS binds to and actively suppresses the expression of the tet-Oregulated TGF-beta1 transgene. In the presence of dox, tTS is released allowing the activating, dox binding rtTA to bind the tet-O and activate transgene expression. (J:92469)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:92469 Lee CG, et al., Early growth response gene 1-mediated apoptosis is essential for transforming growth factor beta1-induced pulmonary fibrosis. J Exp Med. 2004 Aug 2;200(3):377-89
• All: 7 reference(s)