Cdc25a^tm1Hpw
Targeted Allele Detail

Summary

• Symbol: Cdc25a^tm1Hpw
• Name: cell division cycle 25A; targeted mutation 1, Helen Piwnica-Worms
• MGI ID: MGI:3836585
• Synonyms: A^f, Cdc25A^f
• Gene: Cdc25a Location: Chr9:109704647-109722963 bp, + strand Genetic Position: Chr9, 59.81 cM
• Alliance: Cdc25a^tm1Hpw page

Shortened villi in the small intestine of Cdc25a^tm1Hpw/Cdc25a^tm1.1Hpw Cdc25b^tm1Pjd/Cdc25b^tm1Pjd Cdc25c^tm1Hpw/Cdc25c^tm1Hpw Gt(ROSA)26Sor^{tm1(cre/ERT)Brn}/Gt(ROSA)26Sor⁺ (TKO) mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:145768
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A loxP site was introduce 5' of exon 1 and a loxP-flanked selection cassette was introduced to intron 3 via 129X1/SvJ-derived RW4 embryonic stem (ES) cells. ES cell clones containing the recombinant allele were expanded and transiently transfected with a plasmid encoding Cre recombinase under the control of the CMV promoter to remove the selection cassette, but leave floxed exons 1-3. (J:145768)

Generation of the Cdc25a^tm1Hpw and Cdc25a^tm1.1Hpw alleles

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdc25a Mutation: 34 strains or lines available

References

• Original: J:145768 Lee G, et al., Response of small intestinal epithelial cells to acute disruption of cell division through CDC25 deletion. Proc Natl Acad Sci U S A. 2009 Mar 24;106(12):4701-6
• All: 3 reference(s)