Tg(Myh6-AIP/PLN*)46Jded
Transgene Detail

Summary

• Symbol: Tg(Myh6-AIP/PLN*)46Jded
• Name: transgene insertion 46, John R Dedman
• MGI ID: MGI:3836606
• Synonyms: alpha-MHC-AIP4-SR, SR-AIP
• Transgene: Tg(Myh6-AIP/PLN*)46Jded Location: unknown
• Alliance: Tg(Myh6-AIP/PLN*)46Jded page

Tg(Myh6-AIP/PLN*)46Jded/0 females producing at least 3 litters develop cardiac hypertrophy and dilated heart failure

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-AIP/PLN*)46Jded expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Camk2a (MGI:88256)
  mouse	Pln (MGI:97622)

• Mutation details: The alpha-MHC-AIP4-SR transgene was designed with the 5.5-kb murine alpha-myosin heavy chain (alpha-MHC or Myh6) promoter, an AIP4 CaMKII inhibitor domain (encoding a tetramer of the CaMKII autocamtide inhibitory peptide AIP (KKALRRQEAVDAL; a 13-amino acid sequence known to be a highly specific and potent inhibitor of CaMKII)), a FLAG epitope (DYKDDDDK), and a synthetic truncated phospholamban (PLB) transmembrane domain (amino acids 23-52 with L31A/N34A mutations to ensure AIP4 is targeted to the sarcoplasmic reticulum (SR) without the usual inhibition of the PLB transmembrane domain on SERCA activity). This transgene was injected into pronuclei of FVB/N fertilized mouse oocytes, and transgenic offspring were bred to FVB/N to establish founder line 46 (found to carry an estimated 2 copies of the transgene). (J:84333)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:84333 Ji Y, et al., Targeted inhibition of Ca2+/calmodulin-dependent protein kinase II in cardiac longitudinal sarcoplasmic reticulum results in decreased phospholamban phosphorylation at threonine 17. J Biol Chem. 2003 Jul 4;278(27):25063-71
• All: 3 reference(s)