Gpab1^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Gpab1^C57BL/6J
• Name: gp70 autoantibody titers 1; C57BL/6J
• MGI ID: MGI:3836611
• QTL: Gpab1 Location: unknown Genetic Position: Chr9, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased gp70 autoantibody titers in conjunction with BXSB/MpJ alleles at Bxs6. (J:145903)

Notes

Gpab1 interacts with Bxs6 on chromosome 13. C57BL/6J alleles at Gpab1 in conjunction with BXSB/MpJ alleles at Bxs6 enhances gp70 autoantibody production.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:145903

In a BXSB/MpJ x (C57BL/10Ola x BXSB/MpJ)F1 backcross population highly significant linkage to gp70 autoantibody production, LOD=36.7, and gp70 immune complex production, LOD=19.9, mapped to mouse Chromosome 13. A total of 97 polymorphic markers were screened at an average spacing of 20 cM. The QTL, named Bxs6 (BXSB/MpJ autoimmune neprhitis 6), was flanked by markers D13Mit64 and D13Mit233 and peaked at marker D13Mit253 [Fig.3.]. Parental strain BXSB/MpJ displays significantly elevated gp70 titers and gp70 immune complex levelscompared to parental strain C57BL/10Ola. This locus also showed linkage to glomerulonephritis supporting the argument that gp70IC may play an important role in the development of glomerulonephritis .

Homozygosity for BXSB/MpJ-derived alleles at Bsx6 confer increased levels of gp70 immune complex and gp70 autoantibody titers and increased incidence and severity of glomerulonephritis. Gv1 at 36 cM is a previously identified viral antigen locus located near Bxs6. Yaa1 at 35 cM is a gp70 immune complex QTL previously mapped in an NZW x B6 cross that is also located near Bxs6.

Analysis of Bxs6^BXSB/MpJ homozygous animals from the BXSB/MpJ x (C57BL/10Ola x BXSB/MpJ)F1 backcross population detected an interacting locus at 57.9 cM on mouse Chromosome 9 near D9Mit57. This locus is named Gpab1 (gp70autoantibody titers 1). C57BL/6J alleles at Gpab1 derived from the BXSB/MpJ parental strain confer increased gp70 autoantibody titers in conjunction with homozygous BXSB/MpJ alleles at Bxs6.

A separate backcross population of C57BL/10Ola x (C57BL/10Ola x BXSB/MpJ)F1 animals was analyzed and Bxs6 mapped within and overlapped the same interval on chromosome 13 [Fig.3.] with a LOD=6.2 for gp70 autoantibody production, but did not display linkage to glomerulonephritis.

10.21.2015 Curator Note: Because Bxs6 was originally mapped here in the BXSB/MpJ x (C57BL/10Ola x BXSB/MpJ)F1 backcross population we consider the C57BL/10Ola x (C57BL/10Ola x BXSB/MpJ)F1 backcross to be a different mapping population. We consider it to be a separate mapping study and have named the QTL identified in this cross Bxs7.

References

• Original: J:145903 Haywood ME, et al., Autoantigen glycoprotein 70 expression is regulated by a single locus, which acts as a checkpoint for pathogenic anti-glycoprotein 70 autoantibody production and hence for the corresponding development of severe nephritis, in lupus-prone PXSB mice. J Immunol. 2001 Aug 1;167(3):1728-33
• All: 1 reference(s)