Lyn^weeb
Chemically induced Allele Detail

Summary

• Symbol: Lyn^weeb
• Name: LYN proto-oncogene, Src family tyrosine kinase; WeeB
• MGI ID: MGI:3837125
• Synonyms: Lyn^E260G
• Gene: Lyn Location: Chr4:3676865-3791613 bp, + strand Genetic Position: Chr4, 2.05 cM
• Alliance: Lyn^weeb page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Chemically induced (ENU)
• Mutation: Nucleotide substitutions
• Mutation details: Enu mutagenesis caused an A to G transition at nucleotide 864 in exon 8 (GenBank accession number M57696). This converted amino acid 260 from a glutamic acid into a glycine (E260G). This amino acid is immediately C terminal to the GxGxxG G loop necessary for kinase activity for the protein. Mutant protein in homozygote B cells was expressed at slightly higher levels than endogenous protein but it lacks any detectable kinase activity. (J:146161)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lyn Mutation: 65 strains or lines available

References

• Original: J:146161 Barouch-Bentov R, et al., A conserved salt bridge in the G loop of multiple protein kinases is important for catalysis and for in vivo Lyn function. Mol Cell. 2009 Jan 16;33(1):43-52
• All: 2 reference(s)