Mthfd1^Gt(XB175)Byg
Gene trapped Allele Detail

Summary

• Symbol: Mthfd1^Gt(XB175)Byg
• Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase; gene trap XB175, BayGenomics
• MGI ID: MGI:3837220
• Synonyms: Mthfd1^gt, Mthfd1^{gt(pGT0pfs)Stov}
• Gene: Mthfd1 Location: Chr12:76302072-76366577 bp, + strand Genetic Position: Chr12, 33.52 cM, cytoband D2
• Alliance: Mthfd1^Gt(XB175)Byg page

Mutation origin

• Mutant Cell Line: XB175
• Germline Transmission: Earliest citation of germline transmission: J:146083
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs
• Mutation details: The gene trap vector pGT0pfs, wich contains a beta-geo cassette, was inserted between exon 26 and 27. (J:146083)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB175	CC144433 ; (MGI Seq Detail)	5' RACE	Chr12:76358694-76361873 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  55 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Mthfd1^Gt(XB175)Byg allele

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mthfd1 Mutation: 79 strains or lines available

References

• Original: J:146083 MacFarlane AJ, et al., Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism. J Biol Chem. 2009 Jan 16;284(3):1533-9
• All: 7 reference(s)