Drinknacl2^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Drinknacl2^C57BL/6J
• Name: drink sodium chloride 2; C57BL/6J
• MGI ID: MGI:3837336
• QTL: Drinknacl2 Location: unknown Genetic Position: Chr5, Syntenic

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased sodium chloride intake and preference compared to PWK/PhJ. (J:144262)
• Inheritance: Other (see notes)

Notes

Drinknacl2 exhibits additive inheritance.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:144262

Linkage analysis was performed on (C57BL/6J x PWK/PhJ)F2 animals to identify genetic loci associated with preference and intake of calcium and other salt compounds. Animals were subjected to a two-bottle choice test between water and one other solution (CaCl2, CaLa, MgCl2, KCl, NH4Cl, NaCl, citric acid, QHCl, or saccharin) for a period of 96 hours. PWK/PhJ is a high consumer of calcium chloride (CaCl2) and calcium lactate (CaLa) whereas C57BL/6J is a relatively low consumer. PWK/PhJ also consumes more MgCl2 compared to C57BL/6J. However, when consumption of KCl, NaCl and saccharin were analyzed, C57BL/6J animals consumed more of these solutions compared to PWK/PhJ animals. Linkage analysis was performed using 116 polymorphic markers at a resolution of 10cM - 30cM. Thirty QTLs reaching statistical significance were identified. Authors assigned separate QTL nomenclature for each of the salt compound QTL even if they mapped to the same linkage marker.

On mouse Chromosome 5, significant linkage to water intake mapped to 24 Mb near D5Mit348 (LOD=3.4). This locus explains 5.5% of the variance for water intake and is named Drinkwater1 (drink water 1). C57BL/6J-derived alleles at Drinkwater1 confer increased water intake. Linkage to quinine monohydrochloride intake (LOD=3.5), potassium chloride intake (LOD=7.8), and ammonium chloride (LOD=3.7) intake also map to 24 Mb near D5Mit348. These loci are named Drinkqhcl1 (drink quinine monohydrochloride 1), Drinkkcl1 (drink potassium chloride 1), Drinknh4cl1 (drink ammonium chloride 1), respectively. C57BL/6J-derived alleles at Drinkqhcl1, Drinkkcl1 and Drinknh4cl1 confer increased intake with additive inheritance. Drinkqhcl1 explains 3.3% of the variance for QHCl intake, Drinkkcl1 explains 7.5% of the variance for KCl intake, and Drinknh4cl1 explains 3.5% of the variance for NH4Cl intake.

Linkage to KCl preference (LOD=3.9) and saccharin intake (LOD=3.8) mapped to a second locus at 20 cM (36 Mb) near D5Mit352. These QTLs are named Drinkkcl3 (drinking preference for KCl 3) and Drinksac3 (drinking preference for saccharin 3), respectively. C57BL/6J-derived alleles at Drinkkcl3 confer increased preference for KCl while C57BL/6J-derived alleles at Drinksac3 confer increased saccharin intake. Drinkkcl3 explains 3.2% ofthe variance for KCl preference and Drinksac3 explains 12.3% of the variance for saccharin intake.

Linkage to NaCl intake (LOD=12.2) and preference (LOD=8.5) mapped to 34 cM (56 Mb) near rs3713492 and is named Drinknacl2 (drink sodium chloride 2). Thislocus explains 12.6% of the intake variance and 8.5% of the preference variance. C57BL/6J-derived allele at Drinknacl2 confers increased NaCl intake and preference with an additive mode of inheritance.

References

• Original: J:144262 Tordoff MG, et al., Calcium taste preferences: genetic analysis and genome screen of C57BL/6J x PWK/PhJ hybrid mice. Genes Brain Behav. 2008 Aug;7(6):618-28
• All: 1 reference(s)