Mcl1^tm1Yen
Targeted Allele Detail

Summary

• Symbol: Mcl1^tm1Yen
• Name: myeloid cell leukemia sequence 1; targeted mutation 1, Hsin-Fang Yang-Yen
• MGI ID: MGI:3837368
• Synonyms: Mcl-1^SC
• Gene: Mcl1 Location: Chr3:95566099-95570487 bp, + strand Genetic Position: Chr3, 40.74 cM
• Alliance: Mcl1^tm1Yen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146246
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Two upstream promoter elements located at positions -87 (the SIE site) and -70 (the CRE-2 site) were targeted for mutation. The SIE and CRE-2 sites were replaced with BamHI and EcoRI restriction site, respectively, and a floxed neomycin resistance cassette was placed downstream of exon 1. Founder mice were crossed with EIIa-Cre transgenic mice to remove the neomycin cassette. Immunoblot analysis revealed gene expression was reduced by 90% in the thymus but was marginally or not at all affected in various other major organs examined, including liver, heart, lung and spleen. The altered expression was attributed to the lack of SIE and CRE-2 sites because expression was not disturbed in the thymus of mice homozygous for the same loxP insertion but with intact promoter elements. (J:146246)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcl1 Mutation: 35 strains or lines available

References

• Original: J:146246 Yang CY, et al., Promoter knock-in mutations reveal a role of Mcl-1 in thymocyte-positive selection and tissue or cell lineage-specific regulation of Mcl-1 expression. J Immunol. 2009 Mar 1;182(5):2959-68
• All: 1 reference(s)