Gpr65^{Tn(sb-lacZ,GFP)T1.88Jtak}
Transposon induced Allele Detail

Summary

• Symbol: Gpr65^{Tn(sb-lacZ,GFP)T1.88Jtak}
• Name: G-protein coupled receptor 65; transposon insertion T1.88, Junji Takeda
• MGI ID: MGI:3837432
• Synonyms: TDAG8^Tp, TM88 ICR
• Gene: Gpr65 Location: Chr12:98234894-98242903 bp, + strand Genetic Position: Chr12, 49.84 cM
• Alliance: Gpr65^{Tn(sb-lacZ,GFP)T1.88Jtak} page

Mutation origin

• Strain of Origin: mixed

Mutation description

• Allele Type: Transposon induced (Reporter)
• Mutation: Transposon insertion
• Mutation details: Transposition of a single copy of a sleeping beauty transposable element gene trap vector occurred into an exon of this gene. This gene trap vector consists of the inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking human BCL2 splice acceptor sequence, IRES, lacZ, poly A signal, a CAG promoter, GFP, and splice donor sequence. This allele was generated from mice carrying TgTn(sb-lacZ,GFP)T1Jtak and Tg(ACTB-sb10)1Jtak alleles. Gene inactivation was confirmed by RT-PCR analysis from macrophage lysis. (J:146233)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gpr65 Mutation: 22 strains or lines available

References

• Original: J:146233 Mogi C, et al., Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages. J Immunol. 2009 Mar 1;182(5):3243-51
• All: 11 reference(s)