Trim32^{Gt(BGA355)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Trim32^{Gt(BGA355)Byg}
• Name: tripartite motif-containing 32; gene trap BGA355, BayGenomics
• MGI ID: MGI:3837468
• Gene: Trim32 Location: Chr4:65523223-65534475 bp, + strand Genetic Position: Chr4, 34.43 cM, cytoband C1
• Alliance: Trim32^{Gt(BGA355)Byg} page

Mutation origin

• Mutant Cell Line: BGA355
• Germline Transmission: Earliest citation of germline transmission: J:146155
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, containing a beta-geo cassette, inserted at nucleotide 410 within exon 2. The absence of transcript expression was confirmed by RT-PCR analysis on brain extracts. Note transcript expression of the flanking gene is normal as confirmed by RT-PCR analysis on brain and skeletal muscle extracts. (J:146155)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  BGA355	CW509319 ; (MGI Seq Detail)	5' RACE	Chr4:65531559-65531660 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trim32 Mutation: 27 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:146155 Kudryashova E, et al., Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Hum Mol Genet. 2009 Apr 1;18(7):1353-67
• All: 11 reference(s)