Cert1^{Gt(RRF047)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Cert1^{Gt(RRF047)Byg}
• Name: ceramide transporter 1; gene trap RRF047, BayGenomics
• MGI ID: MGI:3837565
• Synonyms: Cert^gt
• Gene: Cert1 Location: Chr13:96679243-96776675 bp, + strand Genetic Position: Chr13, 50.58 cM
• Alliance: Cert1^{Gt(RRF047)Byg} page

Cardiac defects in Cert1^{Gt(RRF047)Byg}/Cert1^{Gt(RRF047)Byg} embryos

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RRF047
• Germline Transmission: Earliest citation of germline transmission: J:146172
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector, pGT2Lxf, was inserted between exons 3 and 4 disrupting the pleckstrin homology domain and resulting in a truncated protein product. The exact site of insertion was 18394 nucleotides downstream of the 5' end of the third intron. RT-PCR analysis failed to detect full length transcript in homozygous embryos. Western blot analysis detected little or no protein expression in homozygous embryos. (J:146172)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRF047	CC249320 ; (MGI Seq Detail)	5' RACE	Chr13:96679575-96708532 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  5 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cert1 Mutation: 28 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:146172 Wang X, et al., Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol. 2009 Jan 12;184(1):143-58
• All: 4 reference(s)