Eef1a1^{tm1(Kras*)Arge}
Targeted Allele Detail

Summary

• Symbol: Eef1a1^{tm1(Kras*)Arge}
• Name: eukaryotic translation elongation factor 1 alpha 1; targeted mutation 1, Argiris Efstratiadis
• MGI ID: MGI:3837679
• Synonyms: Eef1a1-Kras*
• Gene: Eef1a1 Location: Chr9:78385735-78389006 bp, - strand Genetic Position: Chr9, 43.65 cM, cytoband E1
• Alliance: Eef1a1^{tm1(Kras*)Arge} page

Histology and immunophenotyping of mammary carcinomas in Eef1a1^{tm1(Kras*)Arge}/Eef1a1⁺ Wap^tm1(cre)Arge/Wap⁺ mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146290
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Eef1a1^{tm1(Kras*)Arge} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kras (MGI:96680)

• Mutation details: A loxP site flanked neomycin resistance gene and STOP cassette was inserted along with a constitutively active Kras with the amino acid substitution of glycine with aspartic acid at position 12 (p.G12D) into intron 1. Cre-mediated removal of the STOP cassette is required to achieve expression of the constitutively active Kras. (J:146290)

Generation of the Eef1a1^{tm1(Kras*)Arge} allele

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Eef1a1 Mutation: 60 strains or lines available

References

• Original: J:146290 Klinakis A, et al., Igf1r as a therapeutic target in a mouse model of basal-like breast cancer. Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2359-64
• All: 3 reference(s)