Rev3l^tm1Rsky
Targeted Allele Detail

Summary

• Symbol: Rev3l^tm1Rsky
• Name: REV3 like, DNA directed polymerase zeta catalytic subunit; targeted mutation 1, Klaus Rajewsky
• MGI ID: MGI:3837724
• Synonyms: Pol-zeta^f
• Gene: Rev3l Location: Chr10:39608114-39751207 bp, + strand Genetic Position: Chr10, 21.04 cM
• Alliance: Rev3l^tm1Rsky page

Chromosome aberrations in B cells of Rev3l^tm1Rsky/Rev3l^tm1.1Rsky Tg(Cr2-cre)3Cgn/0 mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146453
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: Exon 2 was targeted conditional deletion becuase messenger RNA splicing from exon 1 to exon 3 would result in a frameshift mutation. A floxed neomycin resistance cassette was placed upstream of the exon, and an additional loxP site was inserted downstream of the exon. After targeting, the neomycin cassette was removed by transient expression of Cre recombinase. Genomic PCR confirmed correct targeting of the locus. (J:146453)

Generation of the Rev3l^tm1Rsky and Rev3l^tm1.1Rsky alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rev3l Mutation: 120 strains or lines available

References

• Original: J:146453 Schenten D, et al., Pol zeta ablation in B cells impairs the germinal center reaction, class switch recombination, DNA break repair, and genome stability. J Exp Med. 2009 Feb 16;206(2):477-90
• All: 2 reference(s)